| Enzyme | Sequence | Overhang | Properties | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AatII | G↑ACGT↓C | 3' ACGT | Reaction temp  Reaction buffer  Heat inactivation  sensitive to methlylation  Fast Cut  | |||||||||||
| AccI | GT↓MK↑AC | 5' MK | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| AccIII | T↓CCGG↑A | 5' CCGG | Reaction temp  Reaction buffer  Heat inactivation  sensitive to methlylation  | |||||||||||
| AclI | AA↓CG↑TT | 5' CG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| AcuI | CTGAAGN14↑NN↓ | 3' NN | Reaction temp Reaction buffer Heat inactivation  sensitive to methlylation Fast Cut | |||||||||||
| AflII | C↓TTAA↑G | 5' TTAA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| AgeI | A↓CCGG↑T | 5' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ AgeI | A↓CCGG↑T | 5' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| AlwI | GGATCN4↓N↑ | 5' N | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Alw26I | GTCTCN↓NNNN↑ | 5' NNNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| ApaI | G↑GGCC↓C | 3' GGCC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation 
Fast Cut | |||||||||||
| ApaLI | G↓TGCA↑C | 5' TGCA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| ApoI | R↓AATT↑Y | 5' AATT | Reaction temp  Reaction buffer  Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| AscI | GG↓CGCG↑CC | 5' CGCG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| AvaI | C↓YCGR↑G | 5' YCGR | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| AvaII | G↓GWC↑C | 5' GWC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| AvrII | C↓CTAG↑G | 5' CTAG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BalI | TGG↓↑CCA | Blunt | Reaction temp Reaction buffer  Heat inactivation sensitive to methlylation | |||||||||||
| BamHI | G↓GATC↑C | 5' GATC | Reaction temp Reaction buffer  Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ BamHI | G↓GATC↑C | 5' GATC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BbvI | GCAGCN8↓NNNN↑ | 5' NNNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BclI | T↓GATC↑A | 5' GATC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BglI | GCCN↑NNN↓NGGC | 3' NNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BglII | A↓GATC↑T | 5' GATC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BsaI | GGTCTCN↓NNNN↑ | 5' NNNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| BsaWI | W↓CCGG↑W | 5' CCGG | Reaction temp  Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BsiWI | C↓GTAC↑G | 5' GTAC | Reaction temp  Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BsmBI | CGTCTCN↓NNNN↑ | 5' NNNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BsoBI | C↓YCGR↑G | 5' YCGR | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BspEI | T↓CCGG↑A | 5' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| BsrFI | R↓CCGG↑Y | 5' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BspHI | T↓CATG↑A | 5' CATG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BstYI | R↓GATC↑Y | 5' GATC | Reaction temp Reaction buffer  Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BtsCI | GGATG↑NN↓ | 3' NN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Cfr9I | C↓CCGG↑G | 5' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Cfr10I | R↓CCGG↑Y | 5' CCGG | Reaction temp Reaction buffer  Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Cfr42I | CC↑GC↓GG | 3' GC | Reaction temp Reaction buffer  Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| ClaI | AT↓CG↑AT | 5' CG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| CviAI | ↓GATC↑ | 5' GATC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| DdeI | C↓TNA↑G | 5' TNA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| DpnI | GmA↓↑TC | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| DpnII | ↓GATC↑ | 5' GATC | Reaction temp Reaction buffer  Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| DraI | TTT↓↑AAA | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EagI | C↓GGCC↑G | 5' GGCC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Eco47I | G↓GWC↑C | 5' GWC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| EcoNI | CCTNN↓N↑NNAGG | 5' N | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EcoO109I | RG↓GNC↑CY | 5' GNC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EcoRI | G↓AATT↑C | 5' AATT | Reaction temp Reaction buffer  Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ EcoRI | G↓AATT↑C | 5' AATT | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EcoRV | GAT↓↑ATC | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ EcoRV | GAT↓↑ATC | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EcoT38I | G↑RGCY↓C | 3' RGCY | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Esp3I | CGTCTCN↓NNNN↑ | 5' NNNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| FokI | GGATGN9↓NNNN↑ | 5' NNNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| FseI | GG↑CCGG↓CC | 3' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| FspI | TGC↓↑GCA | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HaeII | R↑GCGC↓Y | 3' GCGC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HaeIII | GG↓↑CC | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HgaI | GACGCN5↓NNNNN↑ | 5' NNNNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HincII | GTY↓↑RAC | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HindII | GTY↓↑RAC | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HindIII | A↓AGCT↑T | 5' AGCT | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HinfI | G↓ANT↑C | 5' ANT | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HinP1I | G↓CG↑C | 5' CG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HpaI | GTT↓↑AAC | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HpaII | C↓CG↑G | 5' CG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HphI | GGTGAN7↑N↓ | 3' N | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Hpy188I | TC↑N↓GA | 3' N | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Hpy99I | ↑CGWCG↓ | 3' CGWCG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| HpyCH4V | TG↓↑CA | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| KasI | G↓GCGC↑C | 5' GCGC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| KpnI | G↑GTAC↓C | 3' GTAC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ KpnI | G↑GTAC↓C | 3' GTAC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Kpn2I | T↓CCGG↑A | 5' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Lsp1109I | GCAGCN8↓NNNN↑ | 5' NNNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| MboI | ↓GATC↑ | 5' GATC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| MboII | GAAGAN7↑N↓ | 3' N | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| MluI | A↓CGCG↑T | 5' CGCG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| MnlI | CCTCN6↑N↓ | 3' N | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| MseI | T↓TA↑A | 5' TA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| MspI | C↓CG↑G | 5' CG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| MspA1I | CMG↓↑CKG | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| MunI | C↓AATT↑G | 5' AATT | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| NaeI | GCC↓↑GGC | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| NcoI | C↓CATG↑G | 5' CATG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ NcoI | C↓CATG↑G | 5' CATG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| NdeI | CA↓TA↑TG | 5' TA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| NgoMIV | G↓CCGG↑C | 5' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| NheI | G↓CTAG↑C | 5' CTAG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ NheI | G↓CTAG↑C | 5' CTAG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation | |||||||||||
| NlaIV | GGN↓↑NCC | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| NotI | GC↓GGCC↑GC | 5' GGCC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ NotI | GC↓GGCC↑GC | 5' GGCC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| NruI | TCG↓↑CGA | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| Nt.BstNBI | GAGTCNNNN↓ | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | ||||||||||||
| PaeR7I | C↓TCGA↑G | 5' TCGA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| PflMI | CCAN↑NNN↓NTGG | 3' NNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| PleI | GAGTCN4↓N↑ | 5' N | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| PluTI | G↑GCGC↓C | 3' GCGC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| PspGI | ↓CCWGG↑ | 5' CCWGG | Reaction temp  Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| PstI | C↑TGCA↓G | 3' TGCA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| PvuI | CG↑AT↓CG | 3' AT | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| PvuII | CAG↓↑CTG | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ PvuII | CAG↓↑CTG | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| RsaI | GT↓↑AC | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| SacI | G↑AGCT↓C | 3' AGCT | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ SacI | G↑AGCT↓C | 3' AGCT | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| SacII | CC↑GC↓GG | 3' GCGC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| SalI | G↓TCGA↑C | 5' TCGA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ SalI | G↓TCGA↑C | 5' TCGA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Sau96I | G↓GNC↑C | 5' GNC | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| SbfI | CC↑TGCA↓GG | 3' TGCA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| ScaI | AGT↓↑ACT | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ ScaI | AGT↓↑ACT | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| SdaI | CC↑TGCA↓GG | 3' TGCA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| SfiI | GGCCN↑NNN↓NGGCC | 3' NNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| SgrAI | CR↓CCGG↑YG | 5' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| SmaI | CCC↓↑GGG | Blunt | Reaction temp  Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| SnaBI | TAC↓↑GTA | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| SpeI | A↓CTAG↑T | 5' CTAG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| SphI | G↑CATG↓C | 3' CATG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ SphI | G↑CATG↓C | 3' CATG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Sse9I | ↓AATT↑ | 5' AATT | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| SspI | AAT↓↑ATT | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| EZ-CleanCut™ SspI | AAT↓↑ATT | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| StuI | AGG↓↑CCT | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| StyD4I | ↓CCNGG↑ | 5' CCNGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
| SwaI | ATTT↓↑AAAT | Blunt | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| TaqI | T↓CG↑A | 5' CG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| TspMI | C↓CCGG↑G | 5' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| Tth111I | GACN↓N↑NGTC | 5' N | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| XbaI | T↓CTAG↑A | 5' CTAG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| XcmI | CCANNNN↑N↓NNNNTGG | 3' N | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| XhoI | C↓TCGA↑G | 5' TCGA | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| XmaI | C↓CCGG↑G | 5' CCGG | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation Fast Cut | |||||||||||
| BslI | CCNN↑NNN↓NNGG | 3'NNN | Reaction temp Reaction buffer Heat inactivation sensitive to methlylation
Fast Cut | |||||||||||
회사명 : 회사명 / 대표 : 대표자명
주소 : OO도 OO시 OO구 OO동 123-45
사업자 등록번호 : 123-45-67890
전화 : 02-123-4567 팩스 : 02-123-4568
통신판매업신고번호 : 제 OO구 - 123호
개인정보관리책임자 : 정보책임자명
