ENZYME FINDER

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ENZYME FINDER

ENZYME FINDER
Find the restriction enzyme of Enzynomics
by name, recognition sequence, overhang, or incubation temperature.
SEARCH FOR
Name
Sequence
Overhang
Temperature
Enzyme Sequence Overhang Properties
AatII
G↑ACGT↓C
3' ACGT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

AccI
GT↓MK↑AC
5' MK

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

AccIII
T↓CCGG↑A
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

AclI
AA↓CG↑TT
5' CG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

AcuI
CTGAAGN14↑NN↓
3' NN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

AflII
C↓TTAA↑G
5' TTAA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

AgeI
A↓CCGG↑T
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ AgeI
A↓CCGG↑T
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

AlwI
GGATCN4↓N↑
5' N

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Alw26I
GTCTCN↓NNNN↑
5' NNNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

ApaI
G↑GGCC↓C
3' GGCC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

ApaLI
G↓TGCA↑C
5' TGCA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

ApoI
R↓AATT↑Y
5' AATT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

AscI
GG↓CGCG↑CC
5' CGCG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

AvaI
C↓YCGR↑G
5' YCGR

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

AvaII
G↓GWC↑C
5' GWC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

AvrII
C↓CTAG↑G
5' CTAG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BalI
TGG↓↑CCA
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

BamHI
G↓GATC↑C
5' GATC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ BamHI
G↓GATC↑C
5' GATC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BbvI
GCAGCN8↓NNNN↑
5' NNNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BclI
T↓GATC↑A
5' GATC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BglI
GCCN↑NNN↓NGGC
3' NNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BglII
A↓GATC↑T
5' GATC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BsaI
GGTCTCN↓NNNN↑
5' NNNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BsaWI
W↓CCGG↑W
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BsiWI
C↓GTAC↑G
5' GTAC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BsmBI
CGTCTCN↓NNNN↑
5' NNNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BsoBI
C↓YCGR↑G
5' YCGR

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BspEI
T↓CCGG↑A
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BsrFI
R↓CCGG↑Y
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BspHI
T↓CATG↑A
5' CATG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BstYI
R↓GATC↑Y
5' GATC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

BtsCI
GGATG↑NN↓
3' NN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Cfr9I
C↓CCGG↑G
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Cfr10I
R↓CCGG↑Y
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Cfr42I
CC↑GC↓GG
3' GC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

ClaI
AT↓CG↑AT
5' CG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

CviAI
↓GATC↑
5' GATC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

DdeI
C↓TNA↑G
5' TNA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

DpnI
GmA↓↑TC
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

DpnII
↓GATC↑
5' GATC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

DraI
TTT↓↑AAA
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EagI
C↓GGCC↑G
5' GGCC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Eco47I
G↓GWC↑C
5' GWC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EcoNI
CCTNN↓N↑NNAGG
5' N

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EcoO109I
RG↓GNC↑CY
5' GNC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EcoRI
G↓AATT↑C
5' AATT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ EcoRI
G↓AATT↑C
5' AATT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EcoRV
GAT↓↑ATC
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ EcoRV
GAT↓↑ATC
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EcoT38I
G↑RGCY↓C
3' RGCY

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Esp3I
CGTCTCN↓NNNN↑
5' NNNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

FokI
GGATGN9↓NNNN↑
5' NNNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

FseI
GG↑CCGG↓CC
3' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

FspI
TGC↓↑GCA
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HaeII
R↑GCGC↓Y
3' GCGC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HaeIII
GG↓↑CC
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HgaI
GACGCN5↓NNNNN↑
5' NNNNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HincII
GTY↓↑RAC
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HindII
GTY↓↑RAC
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HindIII
A↓AGCT↑T
5' AGCT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HinfI
G↓ANT↑C
5' ANT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HinP1I
G↓CG↑C
5' CG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HpaI
GTT↓↑AAC
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HpaII
C↓CG↑G
5' CG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HphI
GGTGAN7↑N↓
3' N

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Hpy188I
TC↑N↓GA
3' N

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Hpy99I
↑CGWCG↓
3' CGWCG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

HpyCH4V
TG↓↑CA
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

KasI
G↓GCGC↑C
5' GCGC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

KpnI
G↑GTAC↓C
3' GTAC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ KpnI
G↑GTAC↓C
3' GTAC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Kpn2I
T↓CCGG↑A
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Lsp1109I
GCAGCN8↓NNNN↑
5' NNNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

MboI
↓GATC↑
5' GATC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

MboII
GAAGAN7↑N↓
3' N

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

MluI
A↓CGCG↑T
5' CGCG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

MnlI
CCTCN6↑N↓
3' N

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

MseI
T↓TA↑A
5' TA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

MspI
C↓CG↑G
5' CG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

MspA1I
CMG↓↑CKG
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

MunI
C↓AATT↑G
5' AATT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

NaeI
GCC↓↑GGC
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

NcoI
C↓CATG↑G
5' CATG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ NcoI
C↓CATG↑G
5' CATG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

NdeI
CA↓TA↑TG
5' TA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

NgoMIV
G↓CCGG↑C
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

NheI
G↓CTAG↑C
5' CTAG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ NheI
G↓CTAG↑C
5' CTAG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

NlaIV
GGN↓↑NCC
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

NotI
GC↓GGCC↑GC
5' GGCC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ NotI
GC↓GGCC↑GC
5' GGCC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

NruI
TCG↓↑CGA
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Nt.BstNBI
GAGTCNNNN↓

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

PaeR7I
C↓TCGA↑G
5' TCGA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

PflMI
CCAN↑NNN↓NTGG
3' NNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

PleI
GAGTCN4↓N↑
5' N

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

PluTI
G↑GCGC↓C
3' GCGC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

PspGI
↓CCWGG↑
5' CCWGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

PstI
C↑TGCA↓G
3' TGCA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

PvuI
CG↑AT↓CG
3' AT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

PvuII
CAG↓↑CTG
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ PvuII
CAG↓↑CTG
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

RsaI
GT↓↑AC
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SacI
G↑AGCT↓C
3' AGCT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ SacI
G↑AGCT↓C
3' AGCT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SacII
CC↑GC↓GG
3' GCGC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SalI
G↓TCGA↑C
5' TCGA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ SalI
G↓TCGA↑C
5' TCGA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Sau96I
G↓GNC↑C
5' GNC

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SbfI
CC↑TGCA↓GG
3' TGCA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

ScaI
AGT↓↑ACT
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ ScaI
AGT↓↑ACT
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SdaI
CC↑TGCA↓GG
3' TGCA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SfiI
GGCCN↑NNN↓NGGCC
3' NNN

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SgrAI
CR↓CCGG↑YG
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SmaI
CCC↓↑GGG
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SnaBI
TAC↓↑GTA
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SpeI
A↓CTAG↑T
5' CTAG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SphI
G↑CATG↓C
3' CATG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ SphI
G↑CATG↓C
3' CATG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Sse9I
↓AATT↑
5' AATT

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SspI
AAT↓↑ATT
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

EZ-CleanCut™ SspI
AAT↓↑ATT
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

StuI
AGG↓↑CCT
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

StyD4I
↓CCNGG↑
5' CCNGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

SwaI
ATTT↓↑AAAT
Blunt

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

TaqI
T↓CG↑A
5' CG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

TspMI
C↓CCGG↑G
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Tth111I
GACN↓N↑NGTC
5' N

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

XbaI
T↓CTAG↑A
5' CTAG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

XcmI
CCANNNN↑N↓NNNNTGG
3' N

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

XhoI
C↓TCGA↑G
5' TCGA

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

XmaI
C↓CCGG↑G
5' CCGG

Reaction temp

Reaction buffer

Heat inactivation

sensitive to methlylation

Fast Cut

Ambiguity Codes

N = A or G or T or C(any) / B = C or G or T (not A) / D = A or G or T (not C) / H = A or C or T (not G) / V = A or C or G (not T)
W = A or T (weak) / S = C or G (storng) / R = A or G (puring) / Y = C or T (pyrimidine) / M = A or C (amino) / K = G or T (keto)

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