Restriction enzmyes recognize short DNA sequences and
cleaves double-stranded DNA at
or near a specific recognition site.
Restriction enzmyes are classified into four types, based on their subunit
structure,
cofactor requirements and specificity of cleavage.
All restriction endonucleases recognize a specific DNA sequence.
The recognition sequence is usually palindromic or
partially palindromic, meaning the base sequence reads
the same backwards and forwards. Restriction enzymes can cleave
double stranded DNA either at the center of both strands to yield
“blunt ends” or at a staggered position leaving
overhangs called “sticky ends”.
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